A novel nano-hydroxyapatite/synthetic polymer/bone morphogenetic protein-2 composite for efficient bone regeneration

BACKGROUND Efficient bone regeneration using recombinant human morphogenetic protein-2 (BMP-2) is needed to reduce side effects caused by high-dose BMP-2 use. The composite material of polylactic acid–polyethene glycol (PLA-PEG) for sustained release and an osteogenic nano-hydroxyapatite (nHAp) can contribute efficient BMP-2. STUDY DESIGN An experimental in vitro vivo study. PURPOSE objective this study investigate the effectiveness a novel PLA-PEG nHAp as carrier METHODS kinetics from composites was investigated ELISA. Thirty-six male Sprague–Dawley rats underwent posterolateral spinal fusion on L4–L5 with three different doses (0 µg [control], 3 [low dose], 10 [high dose]). Weekly µCT results histology manual palpation test at 8 weeks postoperatively were used assessment fusion. RESULTS ELISA demonstrated until day 21. solid 91.6% (11/12) specimens both low- groups. N mice control group attained bony (0%, 0/9). resorbed between 2 4 postoperatively, regenerated mass consisted only newly formed bone. CONCLUSIONS nHAp/PLA-PEG enabled low-dose biodegradable scaffold might regeneration. CLINICAL SIGNIFICANCE This has potential clinical applications (spinal fusion, large defect non-union) enabling formation